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Fig. 4 | Microbial Cell Factories

Fig. 4

From: Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supply

Fig. 4

Production of (R)-3-hydroxybutyrate in E. coli AF1000 a pJBGT3RX, reference cultivation. b pJBGT3RX with excess of glutamate in the medium c pJBGT3RX pBADzwf, in batch mode during ammonium depletion. Symbols refer to cell dry weigh (CDW, filled circles), glucose (Glc, crosses), glutamate (Glu, open squares), ammonium (NH4 +, closed squares), 3HB (open triangles) and acetic acid (HAc, closed triangles). The specific productivity of 3HB has been curve fitted to a 1st order polynomial. The dashed line marks the shift between batch cultivation and nitrogen depletion

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Microbial Cell Factories

ISSN: 1475-2859

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