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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Increasing the production of (R)-3-hydroxybutyrate in recombinant Escherichia coli by improved cofactor supply

Fig. 1

Simplified metabolic scheme showing the two strategies for increased NADPH availability in connection to the 3HB production pathway. The first strategy involves reducing the NADPH expenditure of the cell by providing glutamate as a second nitrogen source to ammonia, allowing a major NADPH sink to be bypassed. The second strategy involves overexpression of glucose-6-phosphate dehydrogenase (zwf) to redirect some of the glycolytic flux to the pentose phosphate pathway, increasing the amount of NADPH synthesized

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Microbial Cell Factories

ISSN: 1475-2859

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