Table 3 Primers used in this study
From: Engineering Escherichia coli for production of 4-hydroxymandelic acid using glucose–xylose mixture
Name | Sequence (5′–3′) |
|---|---|
gap F1 | CCATGGTTTAGGAGGATTACAAAATGCAGAACTTCGAAATCGACTACG |
gap R1 | CGGGATCCCTAACGACGTGCCGCACCGA |
gap F2 | CCCAAGCTTGCGTAATGCTTAGGCACA |
gap R2 | ACTGCTTGTTCTTGTGGCGCCATATATTCCACCAGCTATT |
lacUV5F1 | GGACTAGTATGCAGAACTTCGAAATCGACTACG |
lacUV5R1 | CGGGATCCCTAACGACGTGCCGCACCGA |
lacUV5F2 | CGGGATCCGCGCCCAATACGCAAACCG |
lacUV5R2 | CCCAAGCTTCTAACGACGTGCCGCACCGA |
trcF1 | GGAATTCCATATGCAGAACTTCGAAATCGACTACG |
trc F2 | CGGGATCCCTGCAGCGACTGCACGGTG |
T7 F | GGAATTCCATATGCAGAACTTCGAAATCGACTACG |
T7 R | GGGGTACCCTAACGACGTGCCGCACCGA |
aspC F | CGGACTTCCCTTCTGTAACCATAATGGAACCTCGTCATGATG |
aspC R | GTGTAGGCTGGAGCTGCTTC |
aspC VF | AGCCCGCTTTTCAGCGGGCTTCATTGTTTTTAATGCTTACATG |
aspC VR | GGAATTAGCCATGGTCC |
aspC YZF | CCTGCGTTTTCATCAGTAATAGTTGG |
aspC YZR | CCTTATCCGGCCTACAAAATCG |
GGCGAAGAAGTTGTCCATA | |
TTGAGGCATTTCAGTCAGT |