Table 1 Plasmids used to construct the overexpressed DXP pathway in E. coli Bl21 (DE3)
Gene(s) | Vector | Multiple cloning site | Restriction sites |
|---|---|---|---|
dxr | pCola-Duet-1 | I | NcoI, EcoRI |
dxs | pCola-Duet-1 | II | NdeI, XhoI |
ispD/ispF operon | pCDF-Duet-1 | I | NcoI, EcoRI |
idi | pCDF-Duet-1 | II | NdeI, XhoI |