Table 6 Saccharomyces cerevisiae strains used in this study as source of gDNA for production of PCR templates and host organism for genetic engineering

S288c

MATα SUC2 gal2 mal2 mel flo1 flo8-1 hap1 ho bio1 bio6

Reference strain, source of ABZ2 _S288c http://www.yeastgenome.org/strain/S288C/overview

[41]

AWRI1631

Wine yeast strain, source of ABZ1 _AWRI1631 , ABZ2 _AWRI1631full and ABZ2 _AWRI1631½ http://www.yeastgenome.org/strain/AWRI1631/overview

[42]

Lalvin QA23

Wine yeast strain, source of ABZ1 _QA23 and ABZ2 _QA23 http://www.yeastgenome.org/strain/LalvinQA23/overview

[43]

CEN.PK113-5D

MATa ura3-52 MAL2-8^c SUC2

Background strain used to construct the production strains http://www.yeastgenome.org/strain/CENPK/overview

Euroscarf

PABA0

CEN.PK113-5D Δtrp3::natMX Δaro7::ARO4 ^K229L-kanMX pSP-G1

Indole-3-glycerol-phosphate synthase and chorismate mutase knock-out strain with feedback resistant 3-deoxy-D-arabino-heptulosonate-7-phosphate (DAHP) synthase

This study

PABA1

PABA0 pSP-G1-ABZ1 _AWRI1631 -ABZ2 _AWRI1631 ½

PABA0 strain with aminodeoxychorismate synthase from AWRI1631 and aminodeoxychorismate lyase from AWRI1631 over-expressed

This study

PABA2

PABA0 pSP-G1-ABZ1 _AWRI1631 -ABZ2 _AWRI1631 full

PABA0 strain with aminodeoxychorismate synthase from AWRI1631 and aminodeoxychorismate lyase from AWRI1631 over-expressed

This study

PABA3

PABA0 pSP-G1-ABZ1 _AWRI1631 -ABZ2 _S288c

PABA0 strain with aminodeoxychorismate synthase from AWRI1631 and aminodeoxychorismate lyase from S288c over-expressed

This study

PABA4

PABA0 pSP-G1-ABZ1 _AWRI1631 -ABZ2 _QA23

PABA0 strain with aminodeoxychorismate synthase from AWRI1631 and aminodeoxychorismate lyase from QA23 over-expressed

This study

PABA5

PABA0 pSP-G1-ABZ1 _QA23 -ABZ2 _QA23

PABA0 strain with aminodeoxychorismate synthase from QA23 and aminodeoxychorismate lyase from QA23 over-expressed

This study