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Table 2 Bacterial strains and plasmids used in this work

From: Activation of chloramphenicol biosynthesis in Streptomyces venezuelae ATCC 10712 by ethanol shock: insights from the promoter fusion studies

Bacterial strains

Genotype/phenotype

Source/reference

Escherichia coli DH5α

General cloning host: (luxS supE44 ΔlacU169 (ϕ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1, relA1)

BRL

Escherichia coli ET125671

(pUZ8002)

Mediates conjugative DNA transfer from RP4 oriT with helper plasmid pUZ8002 (KanR, CmR)

Methylation deficient (dam , dcm , hsdM )

[16]

Streptomyces venezuelae ATCC10712 (ISP5230)

Wild type, chloramphenicol and jadomycin B producer (in response to disparate conditions)

ATCC

S. venezuelae JZ1

In-frame deletion of jadR1

This work

S. venezuelae JZ2

cml gene cluster deletion in the JZ1 mutant

This work

S. venezuelae

SOK808::cmlFp

Wild type strain harbouring pSOK808::cmlFp

This work

S. venezuelae SOK808::cmlIp

Wild type strain harbouring pSOK808::cmlIp

This work

S. venezuelae

SOK808::cmlMp

Wild type strain harbouring pSOK808::cmlMp

This work

S. venezuelae

SOK808::cmlRp

Wild type strain harbouring pSOK808::cmlRp

This work

S. venezuelae SOK808::jadJp

Wild type strain harbouring pSOK808::jadJp

This work

S. venezuelae SOK808

Wild type strain harbouring pSOK808

This work

S. venezuelae JZ1

SOK808::cmlFp

JZ1 strain harbouring pSOK808::cmlFp

This work

S. venezuelae JZ1

SOK808::cmlIp

JZ1 strain harbouring pSOK808::cmlIp

This work

S. venezuelae JZ1

SOK808::cmlMp

JZ1 strain harbouring pSOK808::cmlMp

This work

S. venezuelae JZ1

SOK808::cmlRp

JZ1 strain harbouring pSOK808::cmlRp

This work

S. venezuelae JZ1

SOK808::jadJp

JZ1 strain harbouring pSOK808::jadJp

This work

S. venezuelae JZ2

SOK808::cmlFp

JZ2 strain harbouring pSOK808::cmlFp

This work

S. venezuelae JZ2

SOK808::cmlIp

JZ2 strain harbouring pSOK808::cmlIp

This work

S. venezuelae JZ2

SOK808::cmlMp

JZ2 strain harbouring pSOK808::cmlMp

This work

S. venezuelae JZ2

SOK808::cmlRp

JZ2 strain harbouring pSOK808::cmlRp

This work

S. venezuelae JZ2

SOK808::jadJp

JZ2 strain harbouring pSOK808::jadJp

This work

Plasmid

Genotype

Source/reference

pSOK201

pSG5 minimal replicon, AmR, RP4 oriT, ColEI replication origin

[22]

pSOK804

ColEI replication origin, AmR, RP4 oriT, VWB attP and int

[21]

pUWLoriT

pIJ101 minimal replicon, ThioR, AmpR, RP4 oriT, ColEI replication origin, ermE*p

[20]

pSOK806

ColEI replication origin, AmR, RP4 oriT, VWB attP and int, ermE*p

This work

pSOK808

ColEI replication origin, AmR, RP4 oriT, attP, int, ermEp::gusA

This work

pSOKjadD

Suicide plasmid for deletion of the jadR1 gene

This work

pSOKcmlD

Suicide plasmid for deletion of the cml gene cluster

This work

p808cmlFp

ColEI replication origin, AmR, RP4 oriT, attP, int, cmlFp::gusA

This work

p808cmlIp

ColEI replication origin, AmR, RP4 oriT, attP, int, cmlIp::gusA

This work

p808cmlMp

ColEI replication origin, AmR, RP4 oriT, attP, int, cmlMp::gusA

This work

p808cmlRp

ColEI replication origin, AmR, RP4 oriT, attP, int, cmlRp::gusA

This work

p808jadJp

ColEI replication origin, AmR, RP4 oriT, attP, int, jadJp::gusA

This work