Bacterial strains | Genotype/phenotype | Source/reference |
---|---|---|
Escherichia coli DH5α | General cloning host: (luxS supE44 ΔlacU169 (ϕ80 lacZΔM15) hsdR17, recA1, endA1, gyrA96, thi-1, relA1) | BRL |
Escherichia coli ET125671 (pUZ8002) | Mediates conjugative DNA transfer from RP4 oriT with helper plasmid pUZ8002 (KanR, CmR) Methylation deficient (dam − , dcm − , hsdM −) | [16] |
Streptomyces venezuelae ATCC10712 (ISP5230) | Wild type, chloramphenicol and jadomycin B producer (in response to disparate conditions) | ATCC |
S. venezuelae JZ1 | In-frame deletion of jadR1 | This work |
S. venezuelae JZ2 | cml gene cluster deletion in the JZ1 mutant | This work |
S. venezuelae SOK808::cmlFp | Wild type strain harbouring pSOK808::cmlFp | This work |
S. venezuelae SOK808::cmlIp | Wild type strain harbouring pSOK808::cmlIp | This work |
S. venezuelae SOK808::cmlMp | Wild type strain harbouring pSOK808::cmlMp | This work |
S. venezuelae SOK808::cmlRp | Wild type strain harbouring pSOK808::cmlRp | This work |
S. venezuelae SOK808::jadJp | Wild type strain harbouring pSOK808::jadJp | This work |
S. venezuelae SOK808 | Wild type strain harbouring pSOK808 | This work |
S. venezuelae JZ1 SOK808::cmlFp | JZ1 strain harbouring pSOK808::cmlFp | This work |
S. venezuelae JZ1 SOK808::cmlIp | JZ1 strain harbouring pSOK808::cmlIp | This work |
S. venezuelae JZ1 SOK808::cmlMp | JZ1 strain harbouring pSOK808::cmlMp | This work |
S. venezuelae JZ1 SOK808::cmlRp | JZ1 strain harbouring pSOK808::cmlRp | This work |
S. venezuelae JZ1 SOK808::jadJp | JZ1 strain harbouring pSOK808::jadJp | This work |
S. venezuelae JZ2 SOK808::cmlFp | JZ2 strain harbouring pSOK808::cmlFp | This work |
S. venezuelae JZ2 SOK808::cmlIp | JZ2 strain harbouring pSOK808::cmlIp | This work |
S. venezuelae JZ2 SOK808::cmlMp | JZ2 strain harbouring pSOK808::cmlMp | This work |
S. venezuelae JZ2 SOK808::cmlRp | JZ2 strain harbouring pSOK808::cmlRp | This work |
S. venezuelae JZ2 SOK808::jadJp | JZ2 strain harbouring pSOK808::jadJp | This work |
Plasmid | Genotype | Source/reference |
---|---|---|
pSOK201 | pSG5 minimal replicon, AmR, RP4 oriT, ColEI replication origin | [22] |
pSOK804 | ColEI replication origin, AmR, RP4 oriT, VWB attP and int | [21] |
pUWLoriT | pIJ101 minimal replicon, ThioR, AmpR, RP4 oriT, ColEI replication origin, ermE*p | [20] |
pSOK806 | ColEI replication origin, AmR, RP4 oriT, VWB attP and int, ermE*p | This work |
pSOK808 | ColEI replication origin, AmR, RP4 oriT, attP, int, ermEp::gusA | This work |
pSOKjadD | Suicide plasmid for deletion of the jadR1 gene | This work |
pSOKcmlD | Suicide plasmid for deletion of the cml gene cluster | This work |
p808cmlFp | ColEI replication origin, AmR, RP4 oriT, attP, int, cmlFp::gusA | This work |
p808cmlIp | ColEI replication origin, AmR, RP4 oriT, attP, int, cmlIp::gusA | This work |
p808cmlMp | ColEI replication origin, AmR, RP4 oriT, attP, int, cmlMp::gusA | This work |
p808cmlRp | ColEI replication origin, AmR, RP4 oriT, attP, int, cmlRp::gusA | This work |
p808jadJp | ColEI replication origin, AmR, RP4 oriT, attP, int, jadJp::gusA | This work |