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Table 2 Calculated χ2-distributed consistency index h, p value, extracellular fraction of the different metabolites analysed for the protocols with cells separation with a quenching temperature of −20° (i.e. WCS − 20°); with cells separation with a quenching temperature of 4 °C (i.e. WCS + 4°) and without cells separation with quenching temperature of −20° (WOCS − 20 °C) of the different metabolites analysed

From: Quantitative metabolomics of the thermophilic methylotroph Bacillus methanolicus

 

Extracellular fraction in  %

h index

p value

WCS − 20°a

WCS + 4°a

WOCS − 20°b

23PG

71.5

80.4

47.5

1.71

0789

6PG

85.5

87.6

23.0

1.75

0781

ADP

53.9

64.8

14.0

0.03

1000

AMP

81.9

86.3

91.0

1.52

0823

ATP

31.7

54.5

1.0

13.10

0011

Cit

82.3

83.5

24.0

81.67

<0.0001

F1P

63.4

74.0

52.5

5.13

0275

F6P

80.2

87.2

75.0

0.86

0930

FBP

38.2

61.7

10.0

1.21

0876

Fum

98.7

95.5

99.5

1.27

0866

G6P

91.6

93.5

84.5

0.03

1000

M6P

70.2

79.1

57.0

1.22

0875

Mal

92.1

93.1

89.5

4.96

0292

PEP

81.3

84.4

59.5

0.75

0945

P-Ser

70.0

81.4

17.0

0.10

0999

R5P

82.9

87.2

55.5

0.13

0998

S7P

76.9

87.6

47.5

1.65

0801

Shi3P

76.8

83.7

53.5

0.06

1000

  1. The metabolite abbreviations and the raw data are given in Additional file 1
  2. aRatio between concentrations in the washing solution and in the washing solution plus quenched and washed cells separated from the medium by filtration (WS *100)/(WS + QC)
  3. bRatio between concentrations in the culture filtrate and in the whole broth (CF*100)/WB