Fig. 3

Precision of the metabolite quantifications by differential method in B. methanolicus. a Precision of metabolite measurements performed on WB and CF samples. Black dashed line represents the average of WB and CF relative standard error. b Precision of intracellular pool measurements obtained using the differential method (WB-CF) versus the extracellular fraction of each specific metabolite. Red dashed line represents the relative standard deviation threshold for acceptable precision (i.e. 20 %). Fumarate values were not plotted because its RSE were over 400. B1 and B2 state for biological replica 1 and 2. The raw data are given in Additional file 1