Bacterial strain | Description | Reference or source |
---|---|---|
Escherichia coli EPI300™ | F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ − rpsL (StrR) nupG trfA dhfr | Epicentre biotechnologies, Madison, WI |
Escherichia coli DH5α | F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen, Karlsruhe, Germany [23] |
Escherichia coli BL21(DE3) | F− ompT hsdSB (r −B m −B ) gal dcm (DE3) | Novagen, Darmstadt, Germany |
Pseudomonas aeruginosa PAO1 | Wild type strain; Ampr | Holloway et al. [24] |
Agrobacterium tumefaciens NTL4 (pCF218)(pCF372) | Reporter strain for AHL detection; traI::lacZ Tetr Spr | |
Chromobacterium violaceum CV026 | Reporter strain for autoinducer I; mini-Tn5 in cviI | McClean [28] |
Escherichia coli KA197 | Phenylalanine auxotroph λ−, e14-, pheA97, relA1, spoT1, thiE1 | Hoekstra et al. [29] |
Escherichia coli JW2580-1 | Phenylalanine auxotroph F − Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ −, ΔpheA762::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514 | Baba et al. [30] |
Acetobacter nitrogenifigens LMG 23498 | Type strain. Cellulose producer | Dutta et al. [31] |
Acetobacter pasteurianus Ap4 | Strain recovered from healthy grapes microfermentation. Celullose producer | Valera et al. [32] |
Acetobacter syzygii LMG 21419 | Type strain. Cellulose producer | Lisdiyanti et al. [33] |
Acetobacter orientalis LMG 21417 | Type strain. Cellulose producer | Lisdiyanti et al. [33] |
Komagataeibacter europaeus DSM 2004 | Cellulose producer | Leibniz-Institut DSMZ, Germany |
Komagataeibacter hansenii LMG 1524 | Strain recovered from vinegar. Cellulose producer | BCCM LMG Collection, Belgium |
Komagataeibacter rhaeticus LMG 22126 | Type strain. Cellulose producer | Dellaglio et al. [34] |
Komagataeibacter europaeus CECT 8454 | Strain recovered from vinegar. Cellulose producer | This study |
Vectors and constructs | Description | Reference or source |
---|---|---|
CopyControl pCC1FOSâ„¢ | F-factor single-copy origin of replication and the inducible high-copy oriV | Epicentre Biotechnologies, Madison, WI |
pBlueScript II SK (+) | Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA | Stratagene, La Jolla, CA, USA |
pDrive | TA-cloning vector, oriEc, Plac lacZ, AmpR, KanR, T7-promotor | QIAGEN (Hilden, Germany) |
pET21a | Expression vector, lacI, AmpR, T7-promotor, C-terminal His6-tag coding sequence | Novagen, Darmstadt, Germany |
pET21a::gqqA | pET21a containing gqqA gene cloned into NdeI and XhoI restriction sites | This study |
pBBR1MCS-5 | Broad host range expression vector, rep, mob, lacZ, GmR | Kovach et al. [35] |
pBBR1MCS-5::gqqA | pBBR1MCS containing gqqA cloned into BamHI and XhoI restriction sites | This study |