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Table 1 Bacterial strains, constructs and vectors used in this study

From: GqqA, a novel protein in Komagataeibacter europaeus involved in bacterial quorum quenching and cellulose formation

Bacterial strain

Description

Reference or source

Escherichia coli EPI300â„¢

F− mcrA Δ(mrr-hsdRMS-mcrBC) Φ80dlacZΔM15 ΔlacX74 recA1 endA1 araD139 Δ(ara, leu)7697 galU galK λ − rpsL (StrR) nupG trfA dhfr

Epicentre biotechnologies, Madison, WI

Escherichia coli DH5α

F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1

Invitrogen, Karlsruhe, Germany [23]

Escherichia coli BL21(DE3)

F− ompT hsdSB (r −B m −B ) gal dcm (DE3)

Novagen, Darmstadt, Germany

Pseudomonas aeruginosa PAO1

Wild type strain; Ampr

Holloway et al. [24]

Agrobacterium tumefaciens NTL4 (pCF218)(pCF372)

Reporter strain for AHL detection; traI::lacZ Tetr Spr

Fuqua et al., Luo et al. [25–27]

Chromobacterium violaceum CV026

Reporter strain for autoinducer I; mini-Tn5 in cviI

McClean [28]

Escherichia coli KA197

Phenylalanine auxotroph λ−, e14-, pheA97, relA1, spoT1, thiE1

Hoekstra et al. [29]

Escherichia coli JW2580-1

Phenylalanine auxotroph F − Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ −, ΔpheA762::kan, rph-1, Δ(rhaD-rhaB)568, hsdR514

Baba et al. [30]

Acetobacter nitrogenifigens LMG 23498

Type strain. Cellulose producer

Dutta et al. [31]

Acetobacter pasteurianus Ap4

Strain recovered from healthy grapes microfermentation. Celullose producer

Valera et al. [32]

Acetobacter syzygii LMG 21419

Type strain. Cellulose producer

Lisdiyanti et al. [33]

Acetobacter orientalis LMG 21417

Type strain. Cellulose producer

Lisdiyanti et al. [33]

Komagataeibacter europaeus DSM 2004

Cellulose producer

Leibniz-Institut DSMZ, Germany

Komagataeibacter hansenii LMG 1524

Strain recovered from vinegar. Cellulose producer

BCCM LMG Collection, Belgium

Komagataeibacter rhaeticus LMG 22126

Type strain. Cellulose producer

Dellaglio et al. [34]

Komagataeibacter europaeus CECT 8454

Strain recovered from vinegar. Cellulose producer

This study

Vectors and constructs

Description

Reference or source

CopyControl pCC1FOSâ„¢

F-factor single-copy origin of replication and the inducible high-copy oriV

Epicentre Biotechnologies, Madison, WI

pBlueScript II SK (+)

Standard cloning vector (phagemid excised from lambda ZAP). The f1 (+) orientation allows rescue of sense strand ssDNA

Stratagene, La Jolla, CA, USA

pDrive

TA-cloning vector, oriEc, Plac lacZ, AmpR, KanR, T7-promotor

QIAGEN (Hilden, Germany)

pET21a

Expression vector, lacI, AmpR, T7-promotor, C-terminal His6-tag coding sequence

Novagen, Darmstadt, Germany

pET21a::gqqA

pET21a containing gqqA gene cloned into NdeI and XhoI restriction sites

This study

pBBR1MCS-5

Broad host range expression vector, rep, mob, lacZ, GmR

Kovach et al. [35]

pBBR1MCS-5::gqqA

pBBR1MCS containing gqqA cloned into BamHI and XhoI restriction sites

This study