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Table 3 Oligonucleotide primers used in this study

From: Secretory production of a beta-mannanase and a chitosanase using a Lactobacillus plantarum expression system

Construct

Name

Sequence (5′–3′)

BsCsnA_nt

B.subCsnfwNcoIlong

CATGAAAATCAGTATGCAAAAAGCAGATTTTTGG

B.subCsnfwNcoIshort

AAAATCAGTATGCAAAAAGCAGATTTTTGG

6HisXhoIrvlong

TCGAGTCAATGGTGATGGTGATGGTG

6HisXhoIrvshort

GTCAATGGTGATGGTGATGGTG

BsCsn_OmpA & BlManB_OmpA

FlagNcoIfwlong

CATGAAAAAGACAGCTATCGCGATTG

FlagNcoIfwshort

AAAAAGACAGCTATCGCGATTG

6HisXhoIrvlong

TCGAGTCAATGGTGATGGTGATGGTG

6HisXhoIrvshort

GTCAATGGTGATGGTGATGGTG

BlManB_nt

B.liManBfwNcoIlong

CTAGAAAAAAAACATCGTTTGTTCAATCT

B.liManBfwNcoIshort

AAAAAAAACATCGTTTGTTCAATCTTCG

B.liManB6HisXhoIlong

TCGAGTCAATGGTGATGGTGTTCCACGACAGGCGTCA

B.liManB6HisXhoIshort

GTCAATGGTGATGGTGTTCCACGACAGGCGTCA

BsCsnA_noSP

B.SubMatCsnNcoIFwlong

CATGGCGGGACTGAATAAAGATC

B.SubMatCsnNcoIFwshort

GCGGGACTGAATAAAGATCAAAAGC

6HisXhoIrvlong

TCGAGTCAATGGTGATGGTGATGGTG

6HisXhoIrvshort

GTCAATGGTGATGGTGATGGTG

BlManB_noSP

B.LimanBMatfwNcoIlong

CATGGCACACACCGTTTCTCCGGTG

B.LimanBMatfwNcoIshort

GCACACACCGTTTCTCCGGTG

6HisXhoIrvlong

TCGAGTCAATGGTGATGGTGATGGTG

6HisXhoIrvshort

GTCAATGGTGATGGTGATGGTG

  1. The primers used for the construction of each construct by sticky-PCR based method are listed. Note that primers 6HisXhoIrvlong and 6HisXhoIrvshort were used for every construct except for BlManB_nt. The long and short primer pairs were used to generate sticky 5′ or 3′ ends as previously described [28]