Secretory production of a beta-mannanase and a chitosanase using a Lactobacillus plantarum expression system

Table 1 Yields and secretion efficiency for production of BlManB and BsCsnA in L. plantarum WCFS1 harboring various expression constructs based on the pSIP409 vector

Enzyme	Type of SP	Volumetric activity (per liter)			% Secretion efficiency	Specific activity (U/mg)
Enzyme	Type of SP	Broth	Cell extract	Total	% Secretion efficiency	Broth	Cell extract
ManB	Native SP	42 ± 1.3 kU	8 ± 0.2 kU	50 ± 1.5 kU	83.7	139	47
	OmpA SP	16 ± 0.6 kU	7 ± 0.3 kU	23 ± 0.9 kU	71.6	84	39
	No SP	5 ± 0.2 kU	20 ± 0.9 kU	25 ± 1.1 kU	19.0	–	92
CsnA	Native SP	63 ± 0.5 kU	36 ± 1.4 kU	99 ± 1.9 kU	63.7	195	168
	OmpA SP	9 ± 0.03 kU	13 ± 0.1 kU	22 ± 0.13 kU	41.2	90	97
	No SP	1 ± 0.05 kU	15 ± 0.7 kU	16 ± 0.8 kU	6.7	–	65

The data were obtained from the culture harvested at 24 h after induction as described in “Methods” section. Data given are the average of two independent experiments ± their standard deviation. Percentage secretion efficiency was calculated by dividing the enzyme activity in the culture broth by the total enzyme activity (broth + cell extract) ×100

ISSN: 1475-2859