Fig. 6
Production of BlManB and BsCsnA using a food-grade expression system. L. plantarum TLG02 strains, harboring pSIP609/BlManB_nt [(BlManB_nt(alr)] or pSIP609/BsCsnA_nt [(BsCsn_nt (alr)], were cultured in 3-L batch fermentations in MRS medium, containing 20 or 40 g/L glucose as indicated. The upper panels show Coomassie-stained SDS-PAGE gels with culture supernatants collected at various time points (20 µL of sample per lane). The bottom panels show the time course of the cultivation corresponding to the SDS-PAGE samples. The pH of the batch cultivation was kept constant at 6.5. Note that doubling the amount of glucose led to a doubling of OD600, but only to a small increase in enzyme production levels (Table 2)