Fig. 2

Production and secretion of B. licheniformis β-mannanase (BlManB, a) and B. subtilis chitosanase (BsCsnA, b) using pSIP409-type constructs containing the native or the OmpA signal peptide. The recombinant L. plantarum strains were batch-cultured in 3-L vessels with pH control at pH 6.5. After harvesting at various time points, volumetric enzyme activities in both the culture supernatant and the cell lysate were determined as described in the “Methods” section. Data given are the average of two independent experiments ± their standard deviation. The lines connecting the points are drawn for illustration purposes only. Key data, supplemented with data for the constructs without signal peptide are summarized in Table 1