Putative virulence factors of Corynebacterium pseudotuberculosis FRC41: vaccine potential and protein expression

Table 1 Physicochemical properties of the target proteins estimated using ProtParam

Physicochemical property	SpaC	SodC	NanH	PknG
Number of amino acids	796	206	694	749
Molecular weight	85,964.9	21,099.3	74,683.3	83,349.4
Theoretical pI	5.13	5.96	5.05	5.13
Total number of negatively charged residues (Asp + Glu)	96	23	102	101
Total number of positively charged residues (Arg + Lys)	77	17	79	76
Extinction coefficient^a	93,085	4595^b	77,600	81,375
Abs 0.1 % (=1 g/l), assuming all pairs of Cys residues form cystines	1.083	0.218^b	1.039	0.976
Extinction coefficient^a	92,710	4470^b	77,350	81,250
Abs 0.1 % (=1 g/l), assuming all Cys residues are reduced	1.078	0.212^b	1.036	0.975
The estimated half-life
Mammalian reticulocytes, in vitro	30 h	30 h	30 h	30 h
Yeast, in vivo	>20 h	>20 h	>20 h	>20 h
Escherichia coli, in vivo	>10 h	>10 h	>10 h	>10 h
Instability index (II)	28.21 (stable)	19.62 (stable)	32.92 (stable)	38.18 (stable)
Aliphatic index	80.16	71.65	72.58	92.91
Grand average of hydropathicity (GRAVY)	−0.442	−0.245	−0.485	−0.211

^a Extinction coefficients are in units of M⁻¹ cm⁻¹, at 280 nm measured in water
^b This protein does not contain any Trp residues. Experience shows that this could result in more than 10 % error in the computed extinction coefficient

ISSN: 1475-2859