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Table 3 Primers used in this study

From: Subtilisin QK-2: secretory expression in Lactococcus lactis and surface display onto gram-positive enhancer matrix (GEM) particles

Primer

Sequencea (5′–3′)

P1

CTGCCATGGCAATGAAAAAAAAGATTATCTC

P2

GCGCATGCGTCGACCGCATCTTGTTTAGCA

P3

GCGGTACCGCCGGAAAAAGCAGTACAGAAAAGA

P4

TGTCTAGA TTATTGTGCAGCTGCTTGTACGTTG

P5

TGTCTAGA TTA GTGGTGGTGGTGGTGGTGTTGTGCAGCTGCTTGTACGT

P6

GCGGTACCGCTGGTAAATCATCAACTG

P7

TGTCTAGA TTA GTGGTGGTGGTGGTGGTGTTGAGCAGCAGCTTGAACGT

P8

AGGAACAGATTGCGCGTATTCAAGAGCGATGTGATCTTC

P9

GCGCAATCTGTTCCTTACGGCAT

P10

TGTCTAGATTATCACTAGTGGTG

P11

TGTCTAGAGGAAATACTAATTCTGGTGG

P12

GCGAGCTC TTA GTGGTGGTGGTGGTGGTGTGTCAGTACAAGTTTTTGA

P13

GCGAGCTC TTA GTGGTGGTGGTGGTGGTGTTTTATTCGTAGATACTGAC

P14

AGAAGAAGCTGAACCTTTTATTCGTAGATACTGACCA

P15

GGTTCAGCTTCTTCTACAAATTCAG

P16

GCGCATGCGGAAATACTAATTCTGGTGG

P17

GCGGTACCTGTCAGTACAAGTTTTTGACC

  1. aUnderlying (single) indicates the restriction enzyme sites; Bold italics indicates the His6-tag sequence; Stop codons (in italics) were introduced to end the translation; Boldface indicates primer extensions necessary for overlap extension