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Fig. 2 | Microbial Cell Factories

Fig. 2

From: Subtilisin QK-2: secretory expression in Lactococcus lactis and surface display onto gram-positive enhancer matrix (GEM) particles

Fig. 2

Western blotting analysis (a) and fibrinolytic activity analysis (b) of Subtilisin QK-2 expressed in L. lactis. 1 five times concentrated supernatant of noninduced L. lactis NZ3900 (pRF02H); 2 supernatant of induced L. lactis NZ3900 (pRF02H); 3 five times concentrated supernatant of noninduced L. lactis NZ3900 (pRF03H); 4 supernatant of induced L. lactis NZ3900 (pRF03H); 5 five times concentrated supernatant of noninduced L. lactis NZ9000 (pRF03H); 6 supernatant of induced L. lactis NZ9000 (pRF03H); 7 supernatant culture of the Bacillus subtilis QK02. The protein amounts analyzed in western blot were three times that of the fibrinolytic activity analysis

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