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Fig. 5 | Microbial Cell Factories

Fig. 5

From: A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

Fig. 5

Evaluation of a signal sequence library on display levels of nanobody-fused chitinase (ChiA-NB-C-IgAP). a The ChiA-NB-C-IgAP fusion was cloned into a set of nine vectors containing different signal peptides for directing the polypeptide to the periplasm. Fluorescence values were measured in a plate reader. Significant variation among the different peptides was observed, with gIII showing the highest signal. Values are averages of biological triplicates, bars standard error. b Comparison of gIII and pelB signal peptides in the pK backbone. Values are averages of four biological replicates and bars show the standard error

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Microbial Cell Factories

ISSN: 1475-2859

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