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Fig. 4 | Microbial Cell Factories

Fig. 4

From: A nanobody:GFP bacterial platform that enables functional enzyme display and easy quantification of display capacity

Fig. 4

FACS-analysis of Chitinase A-nanobody surface display fusions. Fluorescence was measured by flow cytometry 3 h after rhamnose induction of cells in exponential growth followed by 20 min incubation with purified GFP, and two steps of washing. a Chitinase-nanobody fusions surface displayed with LppOmpA, with (green) and without (red) rhamnose induction. b Chitinase-nanobody fusion displayed with C-IgAP, with (green) and without (red) rhamnose induction. c Overlays of the flow cytometry profiles for all fusions, with rhamnose induction: LppOmpA-NB, LppOmpA-ChiA-NB, LppOmpA-NB-ChiA, NB-C-IgAP, and ChiA-NB-C-IgAP

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Microbial Cell Factories

ISSN: 1475-2859

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