Fig. 3

Functional display of Chitinase A using both display anchors. a Protein schemes for Chitinase A-NB fusions. The ChiA protein was fused either in-between OmpA and NB, or C-terminally to LppOmpA-NB. With the C-IgAP anchor, ChiA was fused N-terminally to NB-C-IgAP. b In-gel fluorescence of rhamnose titrations of chitinase-nanobody fusions, and in-gel fluorescence after addition of proteinase K. The same amount of cells was loaded in each lane. c Whole-cell fluorescence for rhamnose titration of each of the chitinase-nanobody fusions. Values are averages of biological duplicates, error bars are standard errors. d Whole cell fluorescence for LppOmpA constructs with and without ChiA, induced with 1 mM rhamnose. Values are averages of biological duplicates, bars show standard error. e Whole cell fluorescence for C-IgAP constructs with and without ChiA, induced with 1 mM rhamnose. Values are averages of biological triplicates, bars show standard error. f Specific chitinase activity for nanobody-chitinase fusions at two different inducer concentrations, normalised to OD. Values are averages of biological duplicates, bars are standard errors. Lppss lpp signal sequence, pelBss pelB signal sequence, OmpA Outer membrane protein A, ChiA Chitinase A, NB nanobody, C-IgAP C-terminal of IgA protease