Three-steps in one-pot: whole-cell biocatalytic synthesis of enantiopure (+)- and (−)-pinoresinol via kinetic resolution

Table 2 Achieved concentration of pinoresinol 3 and ee values for (+)-3a in the one-pot “one-cell” set-up depending on the energy source added

E. coli cell	Energy source	Concentration of 3 (µM)	ee value of ±3a (%)
C41(DE3) harbouring PsVAO and CgL1	None added	153 ± 40.7	4
	25 g l⁻¹ glycerol	351 ± 27	8
	20 g l⁻¹ d-glucose	375 ± 17	5
C41(DE3) harbouring PsVAO and CgL1 and AtPrR2	None added	42 ± 3	≥99
	25 g l⁻¹ glycerol	308 ± 29	2
	20 g l⁻¹ d-glucose	402 ± 28	0

All reactions yielded 100 % conversion of eugenol 1
Reaction conditions: recombinant E. coli cells resuspended in 10 ml 50 mM KPi-buffer, pH 7.5, 100 µM IPTG (cww = 70 g l⁻¹); 2 % (v/v) DMSO; 2.5 mM of eugenol 1. Reactions were carried out for 24 h at 25 °C, 140 rpm