Fig. 4

Two optimization approaches for the synthesis of enantiopure pinoresinol 3. a Stepwise addition of 1 or 2.5 mM eugenol 1 to resting E. coli cells harbouring PsVAO, CgL1, and AtPrR2; b addition of 10 mM eugenol 1 in a one-pot “two-cell” sequential mode of operation: The substrate was added to resting E. coli cells harbouring PsVAO and CgL1. After 24 h resting E. coli cells harbouring AtPrR2 were added. Intensity of yellow colour indicates eugenol 1 concentration in the reaction