Fig. 1
SDS-PAGE and Western-Blot analysis of reductase gene expressions in different E. coli strains. a Expression of syatprr2 with C-terminal His6-tag; b expression of syfiplr with C-terminal His6-tag; c expression of syfiplr without His6-tag. 1 E. coli BL21(DE3) cells before induction, 2 E. coli BL21(DE3) cells 6 h, 3 E. coli BL21(DE3) cells 24 h, 4 E. coli C41(DE3) cells 6 h, 5 E. coli C41(DE3) cells 24 h, 6 E. coli C43(DE3) cells 6 h, 7 E. coli C43(DE3) cells 24 h, 8 E. coli SHuffle T7 Express cells 6 h, 9 E. coli SHuffle T7 Express cells 24 h, M Molecular weight marker