Skip to main content

Table 2 Growth kinetics and stoichiometry of P. putida KT2440 under different growth-limiting conditions

From: Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol

Limitation D (h−1) Residual ammonium (mg L−1) Residual glycerol (g L−1) YX/S (g g−1) PHA (wt%) YPHA/S (g g−1) Uptake and production rates (mmol gCDW−1 h−1)
Glycerol Malate Succinate CO2
Glycerol 0.044 68.2 ± 4.8 ND < 0.1 0.47 2.5 1.02 ± 0.10 ND < 0.1 ND < 0.1 1.5 ± 0.1
Ammonium 0.044 ND < 0.1 19.9 ± 0.9 0.19 29.7 0.08 2.47 ± 0.15 0.04 ± 0.01 0.24 ± 0.02 3.6 ± 0.1
Glycerol 0.120 35.1 ± 2.7 ND < 0.1 0.51 2.7 2.60 ± 0.06 ND < 0.1 ND < 0.1 3.2 ± 0.1
Ammonium 0.120 ND < 0.1 25.7 ± 0.7 0.34 12.8 0.05 3.71 ± 0.03 0.21 ± 0.03 ND < 0.1 4.2 ± 0.1
  1. Standard deviation (±) from at least two independent experiments
  2. Yx/s was calculated based on the biomass, excluding PHA
  3. ND not detected in the culture broth