Integrated analysis of gene expression and metabolic fluxes in PHA-producing Pseudomonas putida grown on glycerol

Table 2 Growth kinetics and stoichiometry of P. putida KT2440 under different growth-limiting conditions

Limitation	D (h⁻¹)	Residual ammonium (mg L⁻¹)	Residual glycerol (g L⁻¹)	Y_X/S (g g⁻¹)	PHA (wt%)	Y_PHA/S (g g⁻¹)	Uptake and production rates (mmol gCDW⁻¹ h⁻¹)
Limitation	D (h⁻¹)	Residual ammonium (mg L⁻¹)	Residual glycerol (g L⁻¹)	Y_X/S (g g⁻¹)	PHA (wt%)	Y_PHA/S (g g⁻¹)	Glycerol	Malate	Succinate	CO₂
Glycerol	0.044	68.2 ± 4.8	ND < 0.1	0.47	2.5	–	1.02 ± 0.10	ND < 0.1	ND < 0.1	1.5 ± 0.1
Ammonium	0.044	ND < 0.1	19.9 ± 0.9	0.19	29.7	0.08	2.47 ± 0.15	0.04 ± 0.01	0.24 ± 0.02	3.6 ± 0.1
Glycerol	0.120	35.1 ± 2.7	ND < 0.1	0.51	2.7	–	2.60 ± 0.06	ND < 0.1	ND < 0.1	3.2 ± 0.1
Ammonium	0.120	ND < 0.1	25.7 ± 0.7	0.34	12.8	0.05	3.71 ± 0.03	0.21 ± 0.03	ND < 0.1	4.2 ± 0.1

ISSN: 1475-2859