Table 4 Strains used in this study
From: A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis
Strains | Genotype and/or relevant characteristic(s) | Source |
|---|---|---|
E. coli DH5α | F−∆lacU169(Ø80d lacZ∆M15) supE44 hsdR17 recA1 gyrA96 endA1 thi-1 relA1 | Invitrogen |
B. subtilis 168 | trpC2 | Lab stock |
B. subtilis 1A751 | eglS∆102 bglT/bglS∆EV aprE nprE his | BGSC |
B. licheniformis CICC 10181 | Wild-type B. licheniformis, amyL gene | CICC |
G. stearothermophilus ATCC 31195 | Wild-type G. stearothermophilus, amyS gene, bgaB gene | ATCC |
1A751C | 1A751 containing pMA5; Kmr | This work |
1A751R | 1A751 containing pMA5R; Kmr | This work |
1A751RS1 | 1A751 containing pMA5RS1; Kmr | This work |
1A751RS2 | 1A751 containing pMA5RS2; Kmr | This work |
1A751RS3 | 1A751 containing pMA5RS3; Kmr | This work |
1A751RS4 | 1A751 containing pMA5RS4; Kmr | This work |
1A751RT1 | 1A751 containing pMA5RT1; Kmr | Lab stock |
1A751RT2 | 1A751 containing pMA5RT2; Kmr | This work |
1A751RT3 | 1A751 containing pMA5RT3; Kmr | This work |
1A751RT4 | 1A751 containing pMA5RT4; Kmr | This work |
1A751P | 1A751 containing pMA5P; Kmr | This work |
1A751Y | 1A751 containing pMA5Y; Kmr | This work |
1A751S | 1A751∆araR::ParaR-Spe; Sper | Lab stock |
1A751T1 | 1A751S ∆tatAdCd; Sper | This work |
1A751T2 | 1A751S∆tatAdCd ∆tatAyCy; Sper | This work |
1A751T3 | 1A751S∆tatAdCd ∆tatAyCy ΔtatAc; Sper | This work |
1A751T3P | 1A751T3 containing pMA5P; Sper Kmr | This work |
1A751T3Y | 1A751T3 containing pMA5Y; Sper Kmr | This work |
1A751T3R | 1A751T3 containing pMA5R; Sper Kmr | This work |
1A751R1 | 1A751 containing pMA5R1; Kmr | This work |
1A751R2 | 1A751 containing pMA5R2; Kmr | This work |
1A751R3 | 1A751 containing pMA5R3; Kmr | This work |
1A751R4 | 1A751 containing pMA5R4; Kmr | This work |
1A751R5 | 1A751 containing pMA5R5; Kmr | This work |
1A751R6 | 1A751 containing pMA5R6; Kmr | This work |
1A751R7 | 1A751 containing pMA5R7; Kmr | This work |
1A751R8 | 1A751 containing pMA5R8; Kmr | This work |
1A751R9 | 1A751 containing pMA5R9; Kmr | This work |
1A751R10 | 1A751 containing pMA5R10; Kmr | This work |
1A751R11 | 1A751 containing pMA5R11; Kmr | This work |
1A751R12 | 1A751 containing pMA5R12; Kmr | This work |
1A751R13 | 1A751 containing pMA5R13; Kmr | This work |
1A751R14 | 1A751 containing pMA5R14; Kmr | This work |
1A751R15 | 1A751 containing pMA5R15; Kmr | This work |
1A751R16 | 1A751 containing pMA5R16; Kmr | This work |
1A751R17 | 1A751 containing pMA5R17; Kmr | This work |
1A751R18 | 1A751 containing pMA5R18; Kmr | This work |
1A751R16E | 1A751 containing pMA5R16E; Kmr | This work |
1A751R17E | 1A751 containing pMA5R17E; Kmr | This work |
1A751L | 1A751 containing pMA5L; Kmr | Lab stock |
1A751G | 1A751 containing pMA5G; Kmr | Lab stock |