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Fig. 2 | Microbial Cell Factories

Fig. 2

From: A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis

Fig. 2

RDPE is secreted independently on Sec or Tat pathway. a Expression of RDPE fused to Sec or Tat signal peptides in B. subtilis 1A751. 1A751 and 1A751C (1A751 containing pMA5) were regarded as negative controls. 1A751R, 1A751 containing pMA5R (encoding RDPE). b Secretion of RDPE in the strain with deficiency of Tat pathway. 1A751P, 1A751 containing pMA5P (encoding PhoD). 1A751Y, 1A751 containing pMA5Y (encoding YwbN). ΔTat-R, 1A751T3R (encoding RDPE). ΔTat-P, 1A751T3P (encoding PhoD). ΔTat-Y, 1A751T3Y (encoding YwbN)

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