Fig. 1
From: A novel strategy for protein production using non-classical secretion pathway in Bacillus subtilis
Expression and secretion of recombinant RDPE in B. subtilis. a Vector map of the recombinant expression plasmid pMA5R. P HpaII , a widely used promoter from Staphylococcus aureus; RBS, ribosome binding site; ColE1, origin of replication for E. coli; bla, ampicillin resistance; RepB, origin of replication for B. subtilis; neo, kanamycin resistance. rdpe, the gene encoding RDPE. b Enzyme activity of recombinant RDPE in medium and cell fraction with 48Â h incubation. Data represent the mean of three parallel experiments, and error bars represent standard error. c SDS-PAGE analysis of expression of recombinant RDPE in medium and cell fractions by B. subtilis 1A751R at incubation of 48Â h. 1A751 and 1A751C are regarded as the negative controls