Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

Guan, Chengran; Cui, Wenjing; Cheng, Jintao; Zhou, Li; Liu, Zhongmei; Zhou, Zhemin

doi:10.1186/s12934-016-0464-0

Microbial Cell Factories

Table 2 Oligonucleotides used in this study

From: Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

Primer	Nucleotide sequence of primer^{a, b}
P1F	TTTGTT ACTTCCTAGAATATATATTATGTAAACT CTTGATATGGCTTTTTATATGTG
P1R	GCAGAAGTTTACATAATATATATTCTAGGAAGTTCAGTCCTGCTCCTCGGCCACGAAG
P2F	AGTACTCGCTGAAAGTCCTGTTGCTGC
P2R	ATCAAGAGTTTACATAATATATATTCTAGGAAGT AACAAATCTCCTTAATTACAAAGCG
P3F	ACTGA ACTTCCTAGAATATATATTATGTAAACT TCTGCAGTGCAGGCTAGCTTTTTTGTGC
P3R	CCGACGAACAAACCTGCCAGAAGCCC
P11F	ACTTTTCACCCATTTTTCGGTTGACAAAAACATTTTTTTCATTTAAACTGAACGGTA
P11R	TTTAAATGAAAAAAATGTTTTTGTCAACCGAAAAATGGGTGAAAAGTTTCATGCGGG
P12F	ATAAAAACATTTTTTTCATTTATAATGAACGGTAGAAAGATAAAAAATATTGAAA
P12R	TTTTATCTTTCTACCGTTCATTATAAATGAAAAAAATGTTTTTATCACCGAAAAA
P13F	ACTTTTCACCCATTTTTCGGTTGACAAAAACATTTTTTTCATGTAAACTGAACGGTA
P13R	TTTACATGAAAAAAATGTTTTGTCAACCGAAAAATGGGTGAAAAGTTTTCATGCGGG
P14F	ATAAAAACATTTTTTTCATGTATAATGAACGGTAGAAAGATAAAAAATATTGAAA
P14R	TTTTATCTTTCTACCGTTCATTATACATGAAAAAAATGTTTTTATCACCGAAAAA
P15F	ACTTTTCACCCATTTTTCGGTTGACAAAAACATTTTTTTCATGTATAATGAACGGTAGAAAGATAAAAAATATTGAAA
P15R	TTTTATCTTTCTACCGTTCATTATACATGAAAAAAATGTTTTTGTCAACCGAAAAATGGGTGAAAAGTTTCATGCGGG
P16F	TGTTAGTTCATAAGAATTAAAATTTATGAATATAAAGTATAGTGTGTTATACTTGCTGATATGAGAAAATGCGTTGCACATGGGATAAGAAA
P16R	GCATTTTCTCTTTCTTATCCATATCAGCAAGTATAACACACTATACTTTATATTCATAAATTTTAATTCTTATGAACTAACAGCCG
P17F	AAGCTGATATGGATAAGAAAGTTTAAAAGAATTGTGAGCGGGAATACAACAACCAACACCAATTAAAGGAGGAAGACAATGATGAGTAAAGGAGAAGAACTTTTCACTGGAG
P17R	TTCTTCTCCTTTACTCATCATTGTCTTCCTCCTTTAATTGGTGTTGGTTGTTGTATTCCCGCTCACAATTCTTTTAAACTTTCTTATCCATATCAGCTTTTAATTCTTATGAACTAACAGCCG
P18F	TGTTAGTTCATAAGAATTAAAATTGACAAATATAAAGTATAGTGTGTTATAATGCTGATATGGATAAGAAAGAGAAAATGC
P18R	TTTCTTATCCATATCAGCATTATAACACACTATACTTTATATTTGTCAATTTAATTCTTATGAACTAACAGCCGAAATAG
P19F	TGTTAGTTCATAAGAATTAAAATTGACAAATATAAAGTATAGTATGTTATACTTGCTGATATGGATAAGAAA
P19R	CCATATCAGCAAGTATAACATACTATACTTTATATTTGTCAATTTTAATTCTTATGAACT
P28F	CTGCTTATAAAGATTAGGGGAGGTATGACAATGATGAGGAAAAAAACGAAAAACAG
P28R	CCGCACAGATGCGTAAGGAGAAAATACCGCTTATTGTGCAGCTGCTTGTACG
P29F	CTGCTTATAAAGATTAGGGGAGGTATGACAATGATGAAAAAGCTTTTGACTGTC
P29R	CCGCACAGATGCGTAAGGAGAAAATACCGCTTATTTGATATCTTCAAAAATG
P30F	CTTCAATAATATTGAAAAAGGAAGAGTGCGGCCGCATCGACAAAAATGTCATGAA
P30R	CAAAGGGGGAAATGGGATCCGAGCTCCCGCGGTTATTTGTATAGTTCATCCATG

^aHomologous sequences were underlined; dif_{B. subtilis} sequence was shown in bold and the mutant sequences were shown in italic bold
^bThe nucleotide sequence of primers P20R/F, P22R/F, P24R/F and P26R/F were the same as P18R/F; the nucleotide sequence of primers P21R/F, P23R/F, P25R/F and P27R/F were the same as P19R/F

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ISSN: 1475-2859

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