Strains
|
E. coli JM109
|
recA1, endA1, gyrA96, thi-1, hsdR17(rk-mk-), e14−(mcrA
−), supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB+, lacIq, lacZΔM15]
| |
B. subtilis 168
|
trpC2
|
This work
|
BSG1682
|
(B. subtilis 168) Δσ
F
|
This work
|
BSG1683
|
The chromosome of B. subtilis 168 integrated with the fragment containing the P
srfA
and the gfp gene
|
This work
|
BSG1684
|
The chromosome of BSG1682 integrated with the fragment containing the P
srfA
and the gfp gene
|
This work
|
BSG101
|
B. subtilis 168, pBSG03 (P
srfA
-GFP)
|
[18]
|
BSG303
|
BSG1682, pBSG03 (P
srfA
-GFP)
|
This work
|
BSG305
|
BSG1682, pBSG05 (mutP
srfA
-GFP)
|
This work
|
BSG3x
|
BSG1682, pBSGx (Px-GFP)
|
This work
|
BSG328
|
BSG1682, pBSG28 (P23-NK)
|
This work
|
BSG329
|
BSG1682, pBSG29 (P23-AP)
|
This work
|
Plasmids
|
P7Z6
|
zeo
r, bla
r, Cre/lox
|
[40]
|
pUC19
|
pUC origin, P
lac
, Ap
r
|
Takara
|
pAX01
|
P
xylA
, erm
r, bla
r, lacA
|
[45]
|
pAX01-GFP
|
pAX01 containing P
srfA
and GFP
|
This work
|
pET-24a-nk
|
pET-24a containing signal peptide of Bpr and the CDS of NK
|
In our lab
|
pBSG03
|
GFP ligated downstream of P
srfA
|
[18]
|
pBSG04
|
pBSG03 with P
srfA
replaced by P
HpaII
|
[18]
|
pBSG05
|
pBSG03 with mutP
srfA
(mutP
srfA
was referred as P05 in this work)
|
[18]
|
pBSG06
|
pBSG05 with GFP replaced by the sequence containing the intrinsic signal peptide and the CDS of AP
|
[18]
|
pBSG3x
|
The derivatives of pBSG03 containing parallel promoter of Px (from P11 to P27)
|
This work
|
pBSG28
|
Derivative of pBSG23 with GFP replaced by the fragment containing the signal peptide of Bpr and the CDS of NK
|
This work
|
pBSG29
|
Derivative of pBSG23 with GFP replaced by the sequence loading the signal peptide and the CDS of AP
|
This work
|