Table 1 Strains and plasmids used in this study
Strains and plasmids | Relevant properties | Reference |
|---|---|---|
Strains | ||
 E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(rk-mk-), e14−(mcrA −), supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB+, lacIq, lacZΔM15] |  |
 B. subtilis 168 | trpC2 | This work |
 BSG1682 | (B. subtilis 168) Δσ F | This work |
 BSG1683 | The chromosome of B. subtilis 168 integrated with the fragment containing the P srfA and the gfp gene | This work |
 BSG1684 | The chromosome of BSG1682 integrated with the fragment containing the P srfA and the gfp gene | This work |
 BSG101 | B. subtilis 168, pBSG03 (P srfA -GFP) | [18] |
 BSG303 | BSG1682, pBSG03 (P srfA -GFP) | This work |
 BSG305 | BSG1682, pBSG05 (mutP srfA -GFP) | This work |
 BSG3x | BSG1682, pBSGx (Px-GFP) | This work |
 BSG328 | BSG1682, pBSG28 (P23-NK) | This work |
 BSG329 | BSG1682, pBSG29 (P23-AP) | This work |
Plasmids | ||
 P7Z6 | zeo r, bla r, Cre/lox | [40] |
 pUC19 | pUC origin, P lac , Ap r | Takara |
 pAX01 | P xylA , erm r, bla r, lacA | [45] |
 pAX01-GFP | pAX01 containing P srfA and GFP | This work |
 pET-24a-nk | pET-24a containing signal peptide of Bpr and the CDS of NK | In our lab |
 pBSG03 | GFP ligated downstream of P srfA | [18] |
 pBSG04 | pBSG03 with P srfA replaced by P HpaII | [18] |
 pBSG05 | pBSG03 with mutP srfA (mutP srfA was referred as P05 in this work) | [18] |
 pBSG06 | pBSG05 with GFP replaced by the sequence containing the intrinsic signal peptide and the CDS of AP | [18] |
 pBSG3x | The derivatives of pBSG03 containing parallel promoter of Px (from P11 to P27) | This work |
 pBSG28 | Derivative of pBSG23 with GFP replaced by the fragment containing the signal peptide of Bpr and the CDS of NK | This work |
 pBSG29 | Derivative of pBSG23 with GFP replaced by the sequence loading the signal peptide and the CDS of AP | This work |