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Table 1 Strains and plasmids used in this study
| Strains and plasmids | Relevant properties | Reference |
|---|---|---|
| Strains | ||
| E. coli JM109 | recA1, endA1, gyrA96, thi-1, hsdR17(rk-mk-), e14−(mcrA −), supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB+, lacIq, lacZΔM15] | |
| B. subtilis 168 | trpC2 | This work |
| BSG1682 | (B. subtilis 168) Δσ F | This work |
| BSG1683 | The chromosome of B. subtilis 168 integrated with the fragment containing the P srfA and the gfp gene | This work |
| BSG1684 | The chromosome of BSG1682 integrated with the fragment containing the P srfA and the gfp gene | This work |
| BSG101 | B. subtilis 168, pBSG03 (P srfA -GFP) | [18] |
| BSG303 | BSG1682, pBSG03 (P srfA -GFP) | This work |
| BSG305 | BSG1682, pBSG05 (mutP srfA -GFP) | This work |
| BSG3x | BSG1682, pBSGx (Px-GFP) | This work |
| BSG328 | BSG1682, pBSG28 (P23-NK) | This work |
| BSG329 | BSG1682, pBSG29 (P23-AP) | This work |
| Plasmids | ||
| P7Z6 | zeo r, bla r, Cre/lox | [40] |
| pUC19 | pUC origin, P lac , Ap r | Takara |
| pAX01 | P xylA , erm r, bla r, lacA | [45] |
| pAX01-GFP | pAX01 containing P srfA and GFP | This work |
| pET-24a-nk | pET-24a containing signal peptide of Bpr and the CDS of NK | In our lab |
| pBSG03 | GFP ligated downstream of P srfA | [18] |
| pBSG04 | pBSG03 with P srfA replaced by P HpaII | [18] |
| pBSG05 | pBSG03 with mutP srfA (mutP srfA was referred as P05 in this work) | [18] |
| pBSG06 | pBSG05 with GFP replaced by the sequence containing the intrinsic signal peptide and the CDS of AP | [18] |
| pBSG3x | The derivatives of pBSG03 containing parallel promoter of Px (from P11 to P27) | This work |
| pBSG28 | Derivative of pBSG23 with GFP replaced by the fragment containing the signal peptide of Bpr and the CDS of NK | This work |
| pBSG29 | Derivative of pBSG23 with GFP replaced by the sequence loading the signal peptide and the CDS of AP | This work |
