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Table 1 Strains and plasmids used in this study

From: Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis

Strains and plasmids Relevant properties Reference
Strains
 E. coli JM109 recA1, endA1, gyrA96, thi-1, hsdR17(rk-mk-), e14(mcrA ), supE44, relA1, Δ(lac-proAB)/F’ [traD36, proAB+, lacIq, lacZΔM15]  
 B. subtilis 168 trpC2 This work
 BSG1682 (B. subtilis 168) Δσ F This work
 BSG1683 The chromosome of B. subtilis 168 integrated with the fragment containing the P srfA and the gfp gene This work
 BSG1684 The chromosome of BSG1682 integrated with the fragment containing the P srfA and the gfp gene This work
 BSG101 B. subtilis 168, pBSG03 (P srfA -GFP) [18]
 BSG303 BSG1682, pBSG03 (P srfA -GFP) This work
 BSG305 BSG1682, pBSG05 (mutP srfA -GFP) This work
 BSG3x BSG1682, pBSGx (Px-GFP) This work
 BSG328 BSG1682, pBSG28 (P23-NK) This work
 BSG329 BSG1682, pBSG29 (P23-AP) This work
Plasmids
 P7Z6 zeo r, bla r, Cre/lox [40]
 pUC19 pUC origin, P lac , Ap r Takara
 pAX01 P xylA , erm r, bla r, lacA [45]
 pAX01-GFP pAX01 containing P srfA and GFP This work
 pET-24a-nk pET-24a containing signal peptide of Bpr and the CDS of NK In our lab
 pBSG03 GFP ligated downstream of P srfA [18]
 pBSG04 pBSG03 with P srfA replaced by P HpaII [18]
 pBSG05 pBSG03 with mutP srfA (mutP srfA was referred as P05 in this work) [18]
 pBSG06 pBSG05 with GFP replaced by the sequence containing the intrinsic signal peptide and the CDS of AP [18]
 pBSG3x The derivatives of pBSG03 containing parallel promoter of Px (from P11 to P27) This work
 pBSG28 Derivative of pBSG23 with GFP replaced by the fragment containing the signal peptide of Bpr and the CDS of NK This work
 pBSG29 Derivative of pBSG23 with GFP replaced by the sequence loading the signal peptide and the CDS of AP This work