Fig. 5From: Development of an efficient autoinducible expression system by promoter engineering in Bacillus subtilis Overproduction of NK (a) and AP (b) using the P23-mediated expression system. The heterologous proteins NK of and AP were inserted downstream of the P23 promoter, resulting in relative plasmids pBSG28 and pBSG29. The corresponding recombinant strains BSG328 and BSG329 were cultivated and periodically sampled. The bands indicating to the target proteins were markedBack to article page