The amyR-deletion strain of Aspergillus niger CICC2462 is a suitable host strain to express secreted protein with a low background

Table 2 Top 15 most abundant proteins of A. niger CICC2462 identified by shotgun LC–MS/MS

No.	Protein description	GN	Pep count^a	Unique pepcount^b	Cover percent (%)^c
1	Glucoamylase	glaA	1022	19	59.22
2	Acid-stable alpha-amylase (fragment)	amyA	358	15	53.51
3	Extracellular alpha-amylase amyA/amyB	amyA	286	16	41.77
4	Acid alpha-amylase	amyA	236	10	38.22
5	Putative uncharacterized protein An01g10930	An01g10930	85	15	27.98
6	Hydrolysis of the 1	An02g07020	39	8	40.00
7	Probable alpha-galactosidase B	aglB	34	7	25.28
8	Catalases convert 2 H(2)O(2) to O(2) + 2 H(2)O	An01g01550	30	12	26.13
9	Endoglucanase A	eglA	29	5	27.62
10	Aspartic proteinase	An01g00370	28	11	42.49
11	Probable beta-mannosidase A	mndA	26	13	23.85
12	Similarity to isoamyl alcohol oxidase mreA	An03g06270	26	9	24.78
13	Hypothetical protein	An01g14730	23	9	47.06
14	Probable endo-1,4-beta-xylanase C	xlnC	20	7	36.39
15	Probable glucan endo-1,3-beta-glucosidase	eglC	14	4	17.17

^aThe pepcount refers to the number of total peptides assigned to proteins
^bThe unique pepcount refers to the number of different peptides assigned to the proteins
^cThe percentage coverage is defined as the ratio (%) of the protein sequence covered by the matched peptides

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