Table 1 PCR primers used in this study
Primers | Sequence (5′–3′) | Enzyme sits |
|---|---|---|
amyR3SH | ACTAGTGCCCCCTACATCTGCTATT | SpeI |
AAGCTTCCACCACCATCAAAATCAC | HindIII | |
amyR3KX | GGTACCTGCCCCCTACATCTGCTATT | KpnI |
CTCGAGTTCCACCACCATCAAAATCAC | XhoI | |
amyR5 | TCTAGACCAGATGTGCTCAAGGAATG | XbaI |
GGTACCAGAAGCGTAGGCTGAACCAT | KpnI | |
glaA | GACAATGGCTACACCAGCAC | |
AATCACACCAGGAGCAGGAC | ||
amyR | CGAGCCGTTCTCTCAGTTTC | |
TGGTGGCAATCTTGTTGAAG | ||
amyA | GCCCATCTACAAAGACGACA | |
ACATTTCCATCCGAACCAAC | ||
actA | CCACGAGACCACCTTCAACTCCA | |
CCACCGATCCAGACGGAGTACTTGC | ||
creA | CGCAATCACCATTTGTTCAG | |
TGGGAGAGGAAGGAGCAGT |