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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

Fig. 8

Induction profiles for conventional induction with manual addition of IPTG solution and optical induction with cIPTG. End-point FbFP fluorescence of cultures conventionally induced with IPTG (a) or optically induced with cIPTG upon UV-A exposure (b). Colors from blue to red indicate induction conditions that lead to increasing FbFP concentrations. For optical induction 400 µM cIPTG was added at the start of the cultivation. Note the axis break at 400 µM IPTG in so that both induction profiles are scaled equally in the range of 0–400 µM IPTG and 0–40 s UV-A exposure to facilitate comparison (a). Results for higher IPTG concentrations (400–1000 µM) are still shown but do not yield higher FbFP concentrations. Also note the vertical black line at an induction time of 6.5 h that highlights a change in microtiter plate lot. IPTG induction experiments with induction times of 1–6.5 h were performed in 48-FlowerPlates of lot 14xx, all other experiments were performed with plates of lot 15xx. The normalization procedure is described in Additional file 7. Cultivation conditions for E. coli Tuner(DE3)/pRhotHi-2-LacI-EcFbFP: 800 µL Wilms-MOPS mineral medium per well in a 48-FlowerPlate, 30 °C, shaking frequency: 1000 rpm, shaking diameter: 3 mm

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