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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

Fig. 5

Onset of heterologous protein production after induction with cIPTG or IPTG. Fluorescence intensity of ester intermediate and NP-uncaging product (open squares, λEx = 326 nm, λEm = 407 nm) and FbFP fluorescence intensity (circles, λEx = 450 nm, λEm = 495 nm) immediately before and after induction with either cIPTG (a) or IPTG (b). Induction is performed with 0–60 s of UV-A irradiation or 0–400 µM IPTG after 10 h of cultivation. In a more data points per time are shown because fewer wells were monitored in parallel

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