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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Light-induced gene expression with photocaged IPTG for induction profiling in a high-throughput screening system

Fig. 1

LED array for individual illumination of 48 wells in a microtiter plate. High performance UV-A LEDs are mounted onto a heat sink. Emitted light passes a transparent gas-permeable sterile barrier and reaches the subjacent well containing the culture and photocaged-IPTG (cIPTG). A mask ensures that no stray light can enter adjacent wells. IPTG is uncaged and induces formation of a fluorescent reporter protein. Protein and biomass concentrations are monitored through the transparent bottom of the microtiter plate using an in-house constructed BioLector setup. A robotic arm moves an optical fiber which is connected to a fluorescence spectrometer from well to well in rapid succession to allow quasi-continuous and non-invasive measurements during continuous orbital shaking for cultivation

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