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Table 3 Synthesized gene sequences used in this study

From: Deregulation of S-adenosylmethionine biosynthesis and regeneration improves methylation in the E. coli de novo vanillin biosynthesis pathway

Gene (and restriction enzymes)

DNA sequence. Restriction enzyme sites are underlined and start/stop codons are in bold

metA* (NdeI/AatII)

AAAAAACAT ATGCCGATTCGTGTGCCGGACGAGCTACCCGCCGTCAATTTCTTGCGTGAAGAAAACGTCTTTGTGATGACAACTTCTTGTGCGTCTGGTCAGGAAATTCGTCCACTTAAGGTTCTGATCCTTAACCTGATGCCGAAGAAGATTGAAACTGAAAATCAGTTTCTGCGCCTGCTTTCAAACTCACCTTTGCAGGTCGATATTCAGCTGTTGCGCATCGATTCCCGTGAATCGCGCAACACGCCCGCAGAGCATCTGAACAACTTCTACTGTAACTTTGAAGATATTCAGGATCAGAACTTTGACGGTTTGATTGTAACTGGTGCGCCGCTGGGCCTGGTGGAGTTTAATGATGTCGCTTACTGGCCGCAGATCAAACAGGTGCTGGAGTGGTCGAAAGATCACGTCACCTCGACGCTGTTTGTCTGCTGGGCGGTACAGGCCGCGCTCAATATCCTCTACGGCATTCCTAAGCAAACTCGCACCGAAAAACTCTCTGGCGTTTACGAGCATCATATTCTCCATCCTCATGCGCTTCTGACGCGTGGCTTTGATGATTCATTCCTGGCACCGCATTCGCGCTATGCTGACTTTCCGGCAGCGTTGATTCGTGATTACACCGATCTGGAAATTCTGGCAGAGACGGAAGAAGGGGATGCATATCTGTTTGCCAGTAAAGATAAGCGCATTGCCTTTGTGACGGGCCATCCCGAATATGATGCGCAAACGCTGGCGCAGGAATTTTTCCGCGATGTGGAAGCCGGACTAGACCCGGATGTACCGTATAACTATTTCCCGCACAATGATCCGCAAAATACACCGCGAGCGAGCTGGCGTAGTCACGGTAATTTACTGTTTACCAACTGGCTCAACTATTACGTCTACCAGAGCACGCTATACGATCTACGGCACATGAATCCAACGCTGGATTAA GACGTCAAAAAA

cysE* (AatII/XhoI)

AAAAAAGACGTCTAATAAAAGGAGATATACCATGTCGTGTGAAGAACTGGAAATTGTCTGGAACAATATTAAAGCCGAAGCCAGAACGCTGGCGGACTGTGAGCCAATGCTGGCCAGTTTTTACCACGCGACGCTACTCAAGCACGAAAACCTTGGCAGTGCACTGAGCTACATGCTGGCGAACAAGCTGTCATCGCCAATTATGCCTGCTATTGCTATCCGTGAAGTGGTGGAAGAAGCCTACGCCGCTGACCCGGAAATGATCGCCTCTGCGGCCTGTGATATTCAGGCGGTGCGTACCCGCGACCCGGCAAGACCCAAATACTCAACCCCGTTGTTATACCTGAAGGGTTTTCATGCCTTGCAGGCCTATCGCATCGGTCACTGGTTGTGGAATCAGGGGCGTCGCGCACTGGCAATCTTTCTGCAAAACCAGGTTTCTGTGACGTTCCAGGTCGATATTCACCCGGCAGCAAAAATTGGTCGCGGTATCATGCTTGACCACGCGACAGGCATCGTCGTTGGTGAAACGGCGGTGATTGAAAACGACGTATCGATTCTGCAATCTGTGACGCTTGGCGGTACGGGTAAATCTGGTGGTGACCGTCACCCGAAAATTCGTGAAGGTGTGATGATTGGCGCGGGCGCGAAAATCCTCGGCAATATTGAAGTTGGGCGCGGCGCGAAGATTGGCGCAGGTTCCGTGGTGCTGCAACCGGTGCCGCCGCATACCACCGCCGCTGGCGTTCCGGCTCGTATTGTCGGTAAACCAGACAGCGATAAGCCATCAATGGATATGGACCAGCATTTCAACGGTATTAACCATACATTTGAGTATGGGGATGGGATCTAA CTCGAGAAAAAA

sahH Sc (NcoI/NotI)

ATATATCC ATGGGCGCCCCTGCTCAGAATTATAAAATTGCAGATATCTCACTGGCAGCGTTCGGTCGTAAGGAAATTGAATTGGCAGAGCACGAAATGCCAGGCCTGATGGCAATTCGTAAAGCCTATGGCGACGTTCAACCGCTGAAGGGCGCACGTATCGCGGGTTGCTTACACATGACGATCCAAACCGCCGTGCTGATTGAGACATTAGTCGCTTTGGGAGCCGAGGTTACGTGGTCGTCATGCAATATCTACTCGACGCAAGATCATGCGGCAGCGGCAATCGCTGCAAGCGGCGTGCCTGTGTTTGCTTGGAAAGGTGAAACCGAAGAGGAATACCTGTGGTGCATTGAACAGCAGCTGTTTGCATTCAAGGATAACAAAAAATTGAACCTGATTTTGGACGACGGCGGGGATTTAACAACCTTAGTTCACGAGAAACATCCTGAAATGCTGGAGGATTGTTTTGGCCTTTCCGAAGAAACCACCACCGGTGTCCACCACCTCTACCGCATGGTTAAAGAGGGTAAACTTAAAGTCCCGGCTATCAATGTGAACGACTCAGTAACCAAATCCAAATTTGATAACTTATATGGTTGCCGTGAAAGCCTGGTTGACGGGATTAAGCGCGCCACGGATGTTATGCTTGCCGGAAAGGTGGCTGTTGTGGCCGGTTACGGTGACGTGGGTAAAGGATGCGCAGCCGCGTTACGTGGCATGGGTGCACGCGTGTTAGTTACGGAAATTGATCCTATCAATGCCCTGCAGGCCGCGATGGAAGGCTATCAAGTGGTAACAATGGAAGATGCCTCACATATCGGTCAGGTGTTCGTTACGACCACGGGTTGTCGTGATATCATCAACGGGGAGCATTTCATTAACATGCCGGAAGATGCAATTGTTTGTAACATCGGGCATTTCGATATTGAGATCGACGTGGCGTGGCTCAAAGCCAATGCCAAAGAGTGTATTAACATTAAACCGCAGGTAGATCGTTATTTGCTGAGCTCTGGCCGTCATGTCATCCTCCTGGCAAATGGACGCCTTGTGAATCTGGGTTGTGCGACGGGTCACTCGTCCTTCGTCATGAGCTGCTCTTTTTCCAACCAGGTTCTGGCACAAATCGCCCTGTTTAAATCTAATGACAAATCTTTTCGCGAAAAACACATCGAGTTCCAGAAGACCGGTCCCTTCGAAGTCGGGGTTCATGTGTTGCCTAAAATCTTAGATGAGGCAGTCGCTAAATTTCATCTTGGCAACCTGGGTGTCCGTCTGACCAAACTGAGTAAAGTCCAGAGCGAGTACCTGGGTATTCCGGAGGAAGGACCTTTCAAAGCGGATCATTATCGCTATTAA GCGGCCGCAAATTT