Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

van der Woude, Aniek D.; Perez Gallego, Ruth; Vreugdenhil, Angie; Puthan Veetil, Vinod; Chroumpi, Tania; Hellingwerf, Klaas J.

doi:10.1186/s12934-016-0458-y

Microbial Cell Factories

Table 1 List of engineered Synechocystis strains for erythritol production

From: Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

	Genotype	Parent strain	Segregated	Highest measured concentration erythritol [#days—OD730]
VPV2	slr0168::Ptrc:tm1254:cmER-kanR	WT	Yes	No erythritol [24d–19.2]
VPV3	slr0168::Ptrc:tm1254:gcy1p-kanR	WT	Yes	No erythritol [24d–9.2]
SAW029	pVZ-Ptrc:tm1254-Ptrc:cmER-kanR	WT	Plasmid	No erythritol [24d–8.8]
SAW030	pVZ-Ptrc:tm1254-Ptrc:gcy1p-kanR	WT	Plasmid	0.71 mM [35d–12.3]
SEP001	slr0168::Ptrc:tm1254-Ptrc:gcy1p-kanR	WT	Yes	0.1 mM [24d–8.0]
SEP002	slr0168::Ptrc:yidA-Ptrc:gcy1p-kanR	WT	Yes	No erythritol [24d–6.5]
SEP003	slr0168::Ptrc:sll1524-Ptrc:gcy1p-kanR	WT	No	No erythritol [36d–10.7]
SEP004	slr0168::Ptrc:tm1254-Ptrc:alr1-kanR	WT	Yes	No erythritol [36d–17.7]
SEP005	slr0168::Ptrc:yidA-Ptrc:alr1-kanR	WT	Yes	No erythritol [36d–15.6]
SEP006	slr0168::Ptrc:sll1524-Ptrc:alr1-kanR	WT	No	No erythritol [36d–17.6]
SEP007	slr0168::Ptrc:tm1254-Ptrc:gld1-kanR	WT	Yes	0.55 mM [36d–17.6]
SEP007m	slr0168::Ptrc:tm1254-Ptrc:gld1	WT	Yes	0.31 mM [30d–17.6]
SEP008	slr0168::Ptrc:yidA-Ptrc:gld1-kanR	WT	Yes	0.05 mM [23d–17.3]
SEP009	slr0168::Ptrc:sll1524-Ptrc:gld1-kanR	WT	No	No erythritol [28d–14.7]
SEP010	slr0168::Ptrc:tm1254-Ptrc:pc20-kanR	WT	Yes	0.04 mM [28d–22.6]
SEP011	slr0168::Ptrc:yidA-Ptrc:pc20-kanR	–	–	–
SEP012	slr0168::Ptrc:sll1524-Ptrc:pc20-kanR	WT	No	No erythritol [28d–24.4]
SEP013	pAVO-Ptrc:tm1254-Ptrc:gld1-kanR	WT	Plasmid	0.98 mM [30d–19.8]
SEP015	slr0168::Ptrc:tm1254-Ptrc:gld1 pVZ-Ptrc:tm1254-kanR	SEP007 m	Plasmid	0.27 mM [30d–19.5]
SEP016	slr0168::Ptrc:tm1254-Ptrc:gld1 pAVO-PcpcBA:H-tm1254-specR	SEP007 m	Plasmid	1.0 mM [33d–21.2]
SEP017	slr0168::Ptrc:tm1254-Ptrc:gld1 pAVO-PcpcBA:H-yidA-specR	SEP007 m	Plasmid	0.65 mM [33d–22.1]
SEP021	pAVO + -PcpcBA:H-tm1254-Ptrc:gld1-specR	WT	Plasmid	1.45 mM [28d–17.6]
SEP022	phaA::PpsbA2:tktS7002-His-camR pAVO + -PcpcBA:H-tm1254-Ptrc:gld1-specR	SRP005	Plasmid	1.9 mM [28d–12.9]
SEP023	phaA::Ptrc:tktS7002-His-camR pAVO + -PcpcBA:H-tm1254-Ptrc:gld1-specR	SRP006	Plasmid	0.66 mM [28d–12.9]
SEP024	phaA::PpsbA2:pktS7942-His-camR pAVO + -PcpcBA:H-tm1254-Ptrc:gld1-specR	SRP007	Plasmid	2.1 mM [28d–13.6]
SEP025	phaA::Ptrc:pktS7942-His-camR pAVO + -PcpcBA:H-tm1254-Ptrc:gld1-specR	SRP008	Plasmid	1.7 mM [28d–15.0]
SRP005	phaA::PpsbA2:tktS7002-His-camR	WT	Yes	NA
SRP006	phaA::Ptrc:tktS7002-His-camR	WT	Yes	NA
SRP007	phaA::PpsbA2:pktS7942-His-camR	WT	Yes	NA
SRP008	phaA::Ptrc:pktS7942-His-camR	WT	Yes	NA

Strains are listed with their respective phosphatase, including tm1254, yidA or sll1524, and erythrose reductase, including cmER, gcy1p, alr1, gld1 or pc20 (Ptrc1 and PcpcBA indicate the used promoters). Slr0168 and phaA mark the respective genes at which genomic insertion took place, whereas pVZ and pAVO are replicative plasmids
NA not applicable. kanR kanamycin resistance cassette, specR spectinomycin resistance cassette, camR chloramphenicol resistance cassette

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ISSN: 1475-2859

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