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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Genetic engineering of Synechocystis PCC6803 for the photoautotrophic production of the sweetener erythritol

Fig. 6

Increasing flux towards erythrose-4-phosphate. a Schematic representation of the CBB cycle intermediates and the reactions/enzymes affecting the E4P concentration. Abbreviations: Tkt transketolase, Pkt phosphoketolase, Tald transaldolase, FBA fructose-1,6-bisphosphate aldolase, GAP glyceraldehyde-3-phosphate, F6P fructose-6-phosphate, FBP fructose-1,6-bisphosphate, Xu5P xylulose-5-phosphate, S7P seduheptolose-7-phosphate, SBP sedoheptulose-1,7-bisphosphate, R5P ribose-5-phosphate, Ru5P ribulose-5-phosphate, RuBP ribulose-1,5-bisphosphate. b Growth and extracellular erythritol production of wildtype Synechocystis (WT) and mutants SEP021 to SEP025. Cells were grown under moderate = intensity illumination (~30 μE/m2/s) in BG-11, supplemented with 25 mM CAPSO (pH = 9) at 30 °C. Solid symbols represent OD730, whereas open symbols depict the erythritol concentration. Error bars represent SD (n = 2)

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