Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Table 1 Primers used for RT-qPCR

Gene	Primer	Sequence	Amplicon length (bp)
tig	ECtig353F	AGGGTCTGGAAGCGATCGAAG	301
tig	ECtig653R	GGGAAGGTCACGTCGATGGT	301
ibpA	ECibpA85F	CAGAGTAATGGCGGCTACCCT	299
ibpA	ECibpA383R	GCTTCCGGAATCACGCGTTC	299
hscB	EChscB191F	CGTTAATGCGCGCGGAATATTTG	301
hscB	EChscB491R	AGTTGTTCGGCACTGCTTCG	301
hchA	EChchA398F	TCGCGGATGTTGTTGCCAG	300
hchA	EChchA697R	CGCCGAAGTACCAGGTGAGA	300
grpE	ECgrpE278F	ACGAATTGCTGCCGGTGATTG	301
grpE	ECgrpE578R	GCTACAGTAACCATCGCCGC	301
groL	ECgroL583F	TTCGACCGTGGCTACCTGTC	301
groL	ECgroL883R	GGGTTGCGATATCCTGCAGC	301
dnaK	ECdnaK504F	CATCAACGAACCGACCGCAG	300
dnaK	ECdnaK803R	TTTTCTGCCGCTTCTTTCAGGC	300
clpB	ECclpB426F	AATGCGTGGAGGTGAAAGCG	300
clpB	ECclpB725R	ATATCCAGCGCCAGTACCCG	300
clpA	ECclpA287F	AGTCCTCCGGTCGCAATGAG	300
clpA	ECclpA586R	CCAGCTCCTTCTCACGACCA	300
cbpA	ECcbpA477F	GCTGAATGTGAAGATCCCGGC	300
cbpA	ECcbpA776R	ACCAGACCTTTGCCTTTAACGC	300
ihfB ^a	ECihfB9F	GTCAGAATTGATAGAAAGACTTGCCACC	258
ihfB ^a	ECihfB266R	CGATCGCGCAGTTCTTTACCAG	258

ISSN: 1475-2859