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Fig. 8 | Microbial Cell Factories

Fig. 8

From: Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Fig. 8

Conformational protein status and activity of the cell’s quality control. a Smoothed CD spectra from 250 to 205 nm of T22-GFP-H6 produced by different E. coli strains and purified at higher imidazol concentration (P2). Beta sheet structure signal is detected at 218 nm. b Expression of chaperone genes in E. coli KPM335 compared to their expression levels in E. coli BW30270. Relative changes in expression of target genes as quantified by RT-qPCR using the comparative 2−∆∆Ct method [25] and the ihfB gene for normalization were converted into log2 values to display the fold changes in log2 scale. The values represent the means and standard deviations based on duplicate RT-qPCR runs for each of the three independent biological replicates per strain. The statistical significance of the differential expression patterns was analyzed using the paired t test. *P < 0.05; **P < 0.01; ***P < 0.001; ns, not significant. Genes labelled with a dash are those whose expression change (either up- or down-regulation) is coincident with the proteomic analysis of a conventional E. coli strain when entering into the stationary phase (according to data from [26]). The tig, hscB, hchA and cbpA gene products were not monitored in this previous study

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