Fig. 6
In vivo biodistribution of nanoparticles in tumor tissue. a Protein accumulation determined by ex vivo imaging of GFP-emitted fluorescence by representative subcutaneous colorectal cancer tumors grown in mice (measurements performed 5 h after the intravenous administration of a 500 μg nanoparticle dose). b Quantitation of GFP-emitted fluorescence in tumors of the compared groups, expressed as total radiant efficiency (photon/s/cm2/sr/μW/cm2). c Representative micrographs of nanoparticle internalization, as detected by IHC with an anti-GFP antibody, in the tumor cell cytosol and CXCR4 expression observed in tumor cells before nanoparticle injection in representative tumors. d Mean ± S.E. of CXCR4 expression H-score in tumor tissue (measured prior to nanoparticle administration) and GFP H-score (a measure of nanoparticle internalization in tumor cells) of mice belonging to the Origami B, MC4100, KPM335, BW30270 or buffer-treated groups. Note the similar level of CXCR4 expression in tumors among groups and the correlation between tumor-emitted fluorescence and the amount of nanoparticle internalized in tumor cells