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Fig. 1 | Microbial Cell Factories

Fig. 1

From: Structural and functional features of self-assembling protein nanoparticles produced in endotoxin-free Escherichia coli

Fig. 1

Protein production and purification. a Scheme of the modular T22-GFP-H6 building block. Sizes of boxes are only approximate and do not precisely correspond to the length of primary aa sequence. b Western blot of crude cell extracts of T22-GFP-H6-producing strains. MW indicates the molecular weight of selected markers. c His-tag affinity chromatography of T22-GFP-H6 produced in the different E. coli strains. Purification was performed using an imidazole concentration gradient. Buffer B contains 500 mM imidazole. d Maldi-TOF identification of T22-GFP-H6 produced in different E. coli strains and purified by affinity chromatography (P2)

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Microbial Cell Factories

ISSN: 1475-2859

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