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Table 1 Plasmids used and constructed in this study

From: Homogeneity and heterogeneity in amylase production by Bacillus subtilis under different growth conditions

Plasmids

Description

Source of reference

pKTH10

kan, amyQ; pUB110

[33]

pDAM

kan, amyM

AB Enzymes GmbH

pHB201 2.4

Cm, ermC, gfp

AB Enzymes GmbH

pKVM2

tetM; E. coli-Bacillus shuttle vector

AB Enzymes GmbH

pBaSysBioII

spec, amp, gfpmut3

[34]

pDAPamy-gfp

kan; transcriptional P(amy)-gfp fusion

This study

pDAPamy-gfp tet

tetM; transcriptional P(amy)-gfp fusion

This study

pDAamyM-gfp

kan; out-of-frame translational amyM-gfp fusion

This study