Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol

Table 4 Kinetic study of the effect of total organic carbon (TOC) concentration

TOC (g/L)	CDM	PHA	Yield	μ	ρ	Productivity
TOC (g/L)	(g/L)	Content (wt %)	(g PHA/g RCM)	(1/h)	(g PHA/g CDM/h)	[g PHA/(L h)]
TOC 5 g/L
Acinetobacter sp. ASC1	12.0 ± 0.1	15.6 ± 1.7	0.19	0.033	0.007	0.05
Pseudomonas sp. ASC2	12.2 ± 0.2	22.4 ± 2.4	0.29	0.031	0.012	0.08
Enterobacter sp. ASC3	13.0 ± 0.2	18.5 ± 3.2	0.23	0.035	0.009	0.07
Bacillus sp. ASC4	8.7 ± 0.3	33.5 ± 1.2	0.5	0.018	0.018	0.08
TOC 10 g/L
Acinetobacter sp. ASC1	23.5 ± 0.1	54.6 ± 3.4	0.49	0.023	0.014	0.36
Pseudomonas sp. ASC2	32.3 ± 0.3	61.8 ± 3.3	0.65	0.047	0.022	0.55
Enterobacter sp. ASC3	33.1 ± 0.2	47.2 ± 2.2	0.57	0.042	0.01	0.43
Bacillus sp. ASC4	13.2 ± 0.4	47.4 ± 1.5	0.51	0.022	0.017	0.17
TOC 20 g/L
Acinetobacter sp. ASC1	27.3 ± 0.2	23.4 ± 2.7	0.31	0.023	0.014	0.18
Pseudomonas sp. ASC2	24.2 ± 0.1	39.4 ± 1.6	0.58	0.044	0.03	0.27
Enterobacter sp. ASC3	27.3 ± 0.2	30.0 ± 2.2	0.42	0.051	0.02	0.15
Bacillus sp. ASC4	21.8 ± 0.5	33.8 ± 2.4	0.41	0.023	0.023	0.2

Cells were grown in production medium with a carbon to nitrogen ratio of 200 in batch cultivation

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