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Table 3 PHA production by isolated strains grown on glucose, refined glycerol, crude glycerol or cooking oils

From: Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol

Substrate

Acinetobacter sp. ASC1

Pseudomonas sp. ASC2

Enterobacter sp. ASC3

Bacillus sp. ASC4

CDM (g/L)

PHAs (wt %)

CDM (g/L)

PHAs (wt %)

CDM (g/L)

PHAs (wt %)

CDM (g/L)

PHAs (wt %)

Glucose

2.7 ± 0.1

8.2 ± 3.5

2.2 ± 0.1

21.7 ± 2.6

2.6 ± 0.2

16.8 ± 1.9

4.8 ± 0.4

18.9 ± 1.5

Refined glycerol

8.6 ± 0.1

3.7 ± 2.4

10.0 ± 0.2

3.9 ± 0.9

7.2 ± 0.1

14.1 ± 2.7

6.1 ± 0.2

6.7 ± 2.8

Crude glycerol

8.5 ± 0.2

25.4 ± 3.1

10.7 ± 0.1

28.2 ± 1.2

9.8 ± 0.3

33.7 ± 1.3

7.8 ± 0.1

34.4 ± 3.4

Canola oil

9.0 ± 0.1

2.2 ± 3.2

12.1 ± 0.2

2.4 ± 2.0

9.2 ± 0.1

1.8 ± 1.4

9.5 ± 0.2

2.5 ± 1.1

Palm oil

13.3 ± 0.4

14.2 ± 3.5

17.8 ± 0.3

5.5 ± 1.2

12.1 ± 0.1

12.0 ± 2.2

14.6 ± 0.3

12.1 ± 2.0

Soybean oil

5.8 ± 0.3

6.7 ± 2.0

9.7 ± 0.1

5.6 ± 2.2

8.1 ± 0.2

5.3 ± 1.8

6.6 ± 0.1

3.4 ± 1.3

Sunflower oil

8.4 ± 0.1

7.3 ± 2.1

7.2 ± 0.1

2.5 ± 1.8

6.5 ± 0.3

5.5 ± 2.0

7.9 ± 0.1

4.7 ± 0.9

Corn oil

5.9 ± 0.2

4.1 ± 1.7

5.1 ± 0.1

2.6 ± 1.5

5.2 ± 0.1

3.1 ± 1.4

5.8 ± 0.3

2.2 ± 1.0

Grape seed oil

5.3 ± 0.1

7.4 ± 1.9

10.2 ± 0.2

5.6 ± 2.5

6.1 ± 0.1

6.3 ± 1.0

6.2 ± 0.1

5.1 ± 1.3

Olive oil

5.3 ± 0.1

5.0 ± 1.0

5.3 ± 0.1

3.7 ± 0.8

4.3 ± 0.2

2.1 ± 0.8

5.0 ± 0.1

3.2 ± 0.7

Rice bran oil

9.9 ± 0.1

5.1 ± 1.2

17.0 ± 0.2

5.9 ± 1.9

10.1 ± 0.1

4.8 ± 1.1

12.5 ± 0.1

6.0 ± 1.5

Camellia seed oil

5.7 ± 0.1

3.4 ± 1.1

5.5 ± 0.1

3.3 ± 1.1

4.8 ± 0.1

2.9 ± 0.6

3.7 ± 0.1

2.2 ± 0.2

  1. Bacteria were cultured in production medium with 20 g/L carbon source at a carbon to nitrogen ratio of 200 for 72 h at 30 °C on a rotary shaker. The dry cell mass was assayed by GC analysis. All data are expressed as ± SD and represent the mean value of three parallel experiments
  2. CDM cell dry mass (g/L), PHA total PHA content (wt %)