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Table 1 Characterization of isolated PHA producing strains by biochemical tests

From: Medium chain length polyhydroxyalkanoates consisting primarily of unsaturated 3-hydroxy-5-cis-dodecanoate synthesized by newly isolated bacteria using crude glycerol

Biochemical test

Results

ASC1

ASC2

ASC3

ASC4

Motility

−

+

+

+

Oxidase

−

+

−

+

Catalase

+

+

+

+

Indole production

−

−

−

−

Methyl red

−

−

−

−

Voges-Proskauer

−

−

+

−

Citrate (simmons)

+

+

+

+

Triple sugar iron (TSI) reaction

K/K

K/K

A/A

K/A

H2S production (TSI)

−

−

−

−

Lysine decarboxylase

−

−

−

+

Ornithine decarboxylase

+

+

+

+

Urease

−

−

+

+

Nitrate reduction

+

+

+

−

  1. K/K alkaline slant/alkaline butt = peptone was used and no carbohydrates were fermented, A/A acid slant/acid butt = glucose, lactose and sucrose were fermented, K/A alkaline slant/acid butt = only glucose was fermented and peptone was used