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Table 3 Alpha-glucosidase activities, Tps1 activities and the neutral trehalase activities of strains

From: MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

  Alpha-glucosidase activitya (μmol/mg/min) Tps1 activitya (U/g CDW) Neutral trehalase activityb (U/g CDW)
BY14a 2.46 ± 0.25 0.80 ± 0.07 12.28 ± 0.88
BY14a + K 2.45 ± 0.22 0.83 ± 0.10 12.26 ± 0.81
B-NTH1 2.45 ± 0.21 0.82 ± 0.07 8.68 ± 0.74*
B-NTH1 + K 2.45 ± 0.23 0.81 ± 0.09 8.39 ± 0.53*
B + MAL62 4.43 ± 0.37** 1.06 ± 0.10* 12.36 ± 0.93
B-NTH1 + MAL62 3.66 ± 0.32** 1.01 ± 0.11* 8.31 ± 0.61*
  1. Values shown represent at least three independent experiments (data are mean ± SD). Significant difference of the transformants (BY14a + K, B-NTH1,B-NTH1 + K, B + MAL62, B-NTH1 + MAL62) from the parental strain was confirmed by Student’s t-test (**P < 0.01,*P < 0.05, n = 3)
  2. aAlpha-glucosidase activities and Tps1 activities were calculated from the cells grown in cane molasses medium
  3. bNeutral trehalase activities were calculated from the cells prefermentation in LSMLD medium