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Table 3 Alpha-glucosidase activities, Tps1 activities and the neutral trehalase activities of strains

From: MAL62 overexpression and NTH1 deletion enhance the freezing tolerance and fermentation capacity of the baker’s yeast in lean dough

 

Alpha-glucosidase activitya (μmol/mg/min)

Tps1 activitya (U/g CDW)

Neutral trehalase activityb (U/g CDW)

BY14a

2.46 ± 0.25

0.80 ± 0.07

12.28 ± 0.88

BY14a + K

2.45 ± 0.22

0.83 ± 0.10

12.26 ± 0.81

B-NTH1

2.45 ± 0.21

0.82 ± 0.07

8.68 ± 0.74*

B-NTH1 + K

2.45 ± 0.23

0.81 ± 0.09

8.39 ± 0.53*

B + MAL62

4.43 ± 0.37**

1.06 ± 0.10*

12.36 ± 0.93

B-NTH1 + MAL62

3.66 ± 0.32**

1.01 ± 0.11*

8.31 ± 0.61*

  1. Values shown represent at least three independent experiments (data are mean ± SD). Significant difference of the transformants (BY14a + K, B-NTH1,B-NTH1 + K, B + MAL62, B-NTH1 + MAL62) from the parental strain was confirmed by Student’s t-test (**P < 0.01,*P < 0.05, n = 3)
  2. aAlpha-glucosidase activities and Tps1 activities were calculated from the cells grown in cane molasses medium
  3. bNeutral trehalase activities were calculated from the cells prefermentation in LSMLD medium
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Microbial Cell Factories

ISSN: 1475-2859

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