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Fig. 4 | Microbial Cell Factories

Fig. 4

From: New insights into paulomycin biosynthesis pathway in Streptomyces albus J1074 and generation of novel derivatives by combinatorial biosynthesis

Fig. 4

Characterization of genes encoding glycosyltransferases. UPLC chromatograms, monitored at 244 nm, of S. albus J1074 mutant strains altered in paulomycins biosynthesis by inactivation of glycosyltransferase coding genes plm12 (SAM5324) and plm23 (SAM5335). All extracts were generated by culturing mutant strains in R5A liquid medium during 96 h. Labeled peaks correspond to paulomycin A (1), paulomycin B (2), paulomenol A (4), paulomenol B (5), 6-hydroxyl-paulinone (6), (2E)-17-(4′-aminophenyl)-3,11,15-trihydroxy-10,12,14-trimethyl-17-oxo-heptadeca-4,6,8-trienoic acid (7), 2-aminobenzoic acid (anthranilic acid) (8), compound not characterized (9*), N-acetyl-orto-aminobenzoic acid (10), deoxydehydrochorismic acid (11)

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