Fig. 9

Effect of tanshinone IIA (TNIIA) on 11β-HSD1 dependent response in the strains RVY97 and RVY102, treated with FK506. a 11β-HSD1 dependent yEGFP fluorescence of strain RVY97 treated with 4 μM cortisone and with increasing TNIIA concentrations. Increasing concentrations of TNIIA led to a drop of yEGFP fluorescence in the yeast population indicating a progressive loss of 11β-HSD1 activity. The fluorescence of untreated cells (no TNIIA) was set at 1. b 11β-HSD1 dependent yEGFP fluorescence of strain RVY102 treated with 32 μM cortisone and increasing TNIIA concentrations. Increasing concentrations of tanshinone improved the stability of yEGFP in the yeast population due to the loss of 11β-HSD1 activity. Cells treated with 32 μM cortisone and 60 μM TNIIA showed a fluorescent signal comparable to the one of the untreated population (DMSO) indicating a nearly complete absence of yEGFP degradation. tdTomato fluorescence was constant in all samples analysed, and it was not affected by treatments with cortisone and TNIIA (not shown). For data normalization details see “Methods” section. Data shown in a and b represent means ± standard deviations of three independent experiments