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Fig. 6 | Microbial Cell Factories

Fig. 6

From: Yeast-based assays for screening 11β-HSD1 inhibitors

Fig. 6

Effect of carbenoxolone (CBX) on 11β-HSD1 dependent response in the strains RVY102. 11β-HSD1 dependent yEGFP fluorescence of strain RVY102 treated with 1000 μM cortisone and increasing CBX concentrations. Increasing concentration of carbenoxolone improved the stability of yEGFP in the yeast population indicating a progressive loss of enzyme activity. Cells treated with 1000 μM cortisone and 3 μM carbenoxolone showed a fluorescence comparable to the one of the untreated population (DMSO) indicating a complete absence of yEGFP degradation. tdTomato fluorescence was maximum and constant in all samples analysed and was not affected by treatments with cortisone and carbenoxolone (not shown). For data normalization details see “Methods” section. Data shown represent means ± standard deviations of three independent experiments

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Microbial Cell Factories

ISSN: 1475-2859

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