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Fig. 5 | Microbial Cell Factories

Fig. 5

From: Yeast-based assays for screening 11β-HSD1 inhibitors

Fig. 5

Effect of cortisone on the yEGFP degradation in yeast strain RVY102. a Increased cortisone concentrations cause the expression of TEV protease in a time and concentration dependent manner. The TEV protease acts on the reporter protein construct causing degradation of the yEGFP. At both time points tested the yEGFP degradation signal in the cortisone-treated cells was increasing with increasing concentrations of cortisone. The treated RVY102 population was analyzed by flow cytometry (10,000 events) and the results are expressed in percentage of RVY102 cells showing loss of green fluorescence due to yEGFP degradation. In all the samples analysed the tdTomato fluorescence was constant and not affected by the treatment with cortisone. Data shown represent means ± standard deviations of three independent experiments. b RVY102 yeast population treated with DMSO and 1000 μM cortisone. Pictures acquired 6 h upon initiation of the treatment with cortisone showed degradation of yEGFP in part of the population (iii), while the tdTomato signal is constant in both treated and untreated cells (ii)

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